Dr. Xuguo "Joe" Zhou

Xuguo "Joe" Zhou

Insect Toxicology Group
Entomology and Nematology Department
University of Florida
Building 970
Natural Area Dr.
Gainesville, FL 32611-0620

Phone: 1-352-392-1901 Ext. 206 (Office), Ext. 200 (Lab)
Email: joezhou@ufl.edu or joezhou@ifas.ufl.edu
Immigration Status: Permanent Resident

EDUCATION

University of Nebraska-Lincoln	Ph.D.	2002	Entomology / Insect Toxicology
China Agricultural University	M.S.	1997	Entomology / Insect Toxicology
Beijing Agricultural College	B.A.	1991	Plant Protection / Entomology

THESIS AND DISSERTATION

Ph.D. Dissertation:	Characterization of esterase-mediated insecticide resistance in western corn rootworms, Diabrotica virgifera virgifera LeConte.
M.S. Thesis:	Biochemical and toxicological characterization of acetylcholinesteras in cotton bollworm, Helicoverpa armigera (Hübner).
B.A. Honors Thesis:	Influences of atmospheric humidity and soil depth on the emergency of Eurgtoma samsonovi Wass (Hymenoptera: Eurytomidae).

EMPLOYMENT HISTORY

06/2005-present	Senior Biological Scientist in Insect Molecular Biology and Insect Toxicology, University of Florida, Gainesville, FL.
08/2004-05/2005	Post-Doctoral Research Associate in Insect Genomics, Molecular Biology and Toxicology, Entomology and Nematology Department, University of Florida, Gainesville, FL. (Advisor: Dr. Mike E. Scharf)
10/2003-08/2004	Post-Doctoral Research Associate in Insect Molecular Biology and Insect Toxicology, Purdue University, West-Lafayette, IN. (Advisor: Drs. Mike E. Scharf and Gary W. Bennett)
01/2003-09/2003	Post-Doctoral Research Associate in Insect Biochemistry and Insect Toxicology, University of Nebraska-Lincoln, NE. (Advisor: Dr. Blair D. Siegfried)
09/1997-12/2002	Graduate Research Assistant (Ph.D.) in Insect Biochemistry and Insect Toxicology, University of Nebraska-Lincoln, Department of Entomology. (Advisor: Drs. Blair D. Siegfried and Lance J. Meinke)
09/1995-08/1997	Lecturer (which is roughly equivalent to the American assistant professor rank), China Agricultural University, Beijing, China, Department of Entomology.
07/1991-09/1995	Teaching Associate (which is roughly equivalent to the American lecturer rank), China Agricultural University, Beijing, China, Department of Entomology.

SUPPLEMENTARY TRAINING

07-08/03/2002	"Getting started as a successful grant writer and academician" seminar, presented by Drs. Stephen Russell and David Morrison of Grant Writers' Seminars and Workshops, LLC.
06/2001-12/2001	Protein purification and characterization, under the direction of facility director Dr. Gautam Sarath, Proteomics Core Facility, University of Nebraska-Lincoln, NE.

TEACHING EXPERIENCE

Toxins in the Environment	University of Nebraska, Entomology 300: Selected Lectures and Grading. 2001-2002.
Insecticide Toxicology	University of Nebraska, Entomology 821: Selected Laboratories and Grading. 1998-2000.
General Entomology	China Agricultural University, Coordinated laboratories and field trips, gave laboratory lectures, and grading. 1991-1995.
Horticultural Entomology	China Agricultural University, Lectures, Field Trips, Laboratories, and Grading. 1991-1997.
Agricultural Entomology	China Agricultural University, Lectures, Field Trips, Laboratories, and Grading. 1991-1997.

PUBLICATIONS

REFEREED JOURNAL PAPERS

Zhou, X.G., M. M. Wheeler, F.M. Oi and M.E. Scharf. 2007. Inhibition of termite cellulases by carbohydrate-based cellulase inhibitors: evidence from in vitro biochemistry and in vivo feeding studies. Pesticide Biochem. Physiol. In press
Zhou, X.G., J.A. Smith, P.G. Koehler, F.M. Oi, G.W. Bennett and M.E. Scharf. 2007. Correlation of cellulase gene expression and cellulolytic activity throughout the gut of the termite Reticulitermes flavipes. Gene. 395: 29-39
Zhou, X.G., M.R. Tarver, and M.E. Scharf. 2007. Hexamerin-based regulation of juvenile hormone-dependent gene expression underlies phenotypic plasticity in a social insect. Development. 134: 601-610.
Kuruganti, S., Lam V., X. Zhou, G.W. Bennet, B.R. Pittendrigh, and R. Ganguly. 2007. High expression of Cyp6g1, a cytochrome P450 gene, does not necessarily confer DDT resistance in Drosophila melanogaster. Gene. 388: 43-53.
Zhou, X.G., C. Song, T. L. Grzymala, F.M. Oi, and M.E. Scharf.2006. Juvenile hormone and colony conditions differentially influence cytochrome P450 gene expression in the termite Reticulitermes flavipes. Insect Mol. Biol. 15: 749-761.
Zhou, X.G., M.R. Tarver, G.W. Bennett, F.M. Oi and M.E. Scharf. 2006. Two hexamerin genes from the termite Reticulitermes flavipes: sequence, expression and proposed functions in caste regulation. GENE: 376:47-58.
Zhou, X.G., F.M. Oi and M.E. Scharf. 2006. Social exploitation of hexamerin: RNAi reveals a major caste-regulatory factor in termites. Proc. Nat. Acad. Sci. USA (TRACK II) 103:4499-4504.
Nowatzki, T. M., X. Zhou, L. J. Meinke, T. Vaughn, and B. D. Siegfried. 2006. Effect of Bacillus thuringiensis Cry3Bb1 Protein on the Feeding Behavior and Longevity of Adult Western Corn Rootworms (Coleoptera: Chrysomelidae). J. Econ. Entomol. 99:927-930.
Sun, L. J., X. Zhou, and X. W. Gao. 2005. Polymorphisms in a carboxylesterase gene between organophosphate resistant and susceptible cotton aphids, Aphis gossypii (Hemiptera: Aphididae). J. Econ. Entomol. 98:1325-1332.
Scharf, M. E., C. R. Ratliff, D. Wu-Scharf, X. Zhou, B.R. Pittendrigh and G.W. Bennet. 2005. Effects of juvenile hormone III on Reticulitermes flavipes: changes in hemolymph protein composition and gene expression. Insect Biochem. Mol. Bio. 35:207-215.
Scharf, M. E., D. Wu-Scharf, X. Zhou, B.R. Pittendrigh and G.W. Bennet. 2005. Gene expression profiles among immature and adult reproductive castes of the termite Reticulitermes flavipes. Insect Mol. Bio. 14:31-44.
Zhou, X., M.E. Scharf, L.J. Meinke, L.D. Chandler and B.D. Siegfried. 2005. Immunological assessment of an insecticide resistance-associated esterase in the western corn rootworm. Arch. Insect Biochem. Physiol. 58:157-165.
Siegfried B.D., L. J. Meinke, S. Parimi, M.E. Scharf, T. J. Nowatzki, X. Zhou and L.D. Chandler. 2004. Monitoring western corn rootworm (Coleoptera: Chrysomelidae) susceptibility to carbaryl and cucurbitacin baits in the area-wide management pilot program. J. Econ. Entomol. 97:1726-1733.
Zhou, X., M.E. Scharf, G. Sarath, L.J. Meinke, L.D. Chandler and B.D. Siegfried. 2004. Partial purification and characterization of a methyl-parathion resistance-associated general esterase in Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae). Pestic. Biochem. Physiol. 78: 114-125.
Zhou, X., M.E. Scharf, L. J. Meinke, L. D. Chandler, and B. D. Siegfried. 2003. Characterization of general esterases from methyl-parathion resistant and susceptible populations of western corn rootworms, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae). J. Econ. Entomol. 96: 1855-1863.
Zhou, X., M.E. Scharf, S. Parimi, L. J. Meinke, R. J. Wright, L. D. Chandler, and B. D. Siegfried. 2002. Diagnostic assays based on esterase-mediated resistance mechanisms in western corn rootworms (Coleoptera: Chrysomelidae). J. Econ. Entomol. 95: 1261-1266.
Gao, X. W., X. Zhou, and B. Z. Zheng. 2001. A comparison of sensitivity to inhibitor among acetylcholinesterase (AChE) molecular forms of resistant and susceptible strains in Helicoverpa armigera. Entomol. Sin. 8: 49-54.
Wright, R. J., M. E. Scharf, L. J. Meinke, X. Zhou, B. D. Siegfried, and L. D. Chandler. 2000. Larval susceptibility of an insecticide-resistant western corn rootworm (Coleoptera: Chrysomelidae) population to soil insecticides: laboratory bioassays, assays of detoxification enzymes, and field performance. J. Econ. Entomol. 93: 7-13.
Gao, X. W., X. Zhou, and B. Z. Zheng.1999. The relationship between acetylcholinesterase molecular forms and insecticides in Helicoverpa armigera. World Pesticide. 21 (suppl.): 108-109.
Gao, X. W., X. Zhou, and B. Z. Zheng. 1998. Body distribution and partial purification of acetylcholinesterase in Helicoverpa armigera. Acta Entomol. Sin. 41 (Suppl.): 19-25.

REFEREED PROCEEDINGS/CONFERENCE PAPERS

Scharf, M.E., X. Zhou and G.W. Bennett. 2005. The application of molecular genomics in addressing long-standing questions on termite biology. Proceedings of 5th International Conference on Urban Pests. Singapore.
Meinke, L. J., M.E. Scharf, S. Parimi, X. Zhou, R. J. Wright, T. J. Nowatzki, L.D. Chandler, and M. A. Caprio. 2003. Developing functional resistance management programs: Lessons learned from prior mistakes. Abstracts of papers of the American Chemical Society 225: U96-U96 0036-AGRO Part 1, Washington, DC.
Siegfried, B. D., L. Meinke, M. E. Scharf, X. Zhou, R. Wright, T. Nowatzki, L. D. Chandler, and M. A. Caprio. 2002. Organophosphate resistance in western corn rootworms: a model for development of future resistance management strategies? Abstracts of 10th International Congress of pesticide Chemistry, #73, IUPAC, Basel, Switzerland.
Siegfried, B. D., M. E. Scharf, X. Zhou, L. Meinke, R. Wright, and L. D. Chandler. 1999. Mechanisms of resistance to organophosphate and carbamate insecticides in western corn rootworm. Abstracts of the XIVth International Congress of Plant Protection, #146, IPPC, Jerusalem, Israel.
Gao, X. W., X. Zhou, and B. Z. Zheng. 1999. Sensitivity of acetylcholinesterase molecular forms to insecticides in Helicoverpa armigera, pp. 724-727. In 1998 Proceeding of Plant Protection in China. China Agricultural Press.

INVITED REVIEWS

Scharf, M.E., X. Zhou, and M. A. Schwinghammer. 2007. Application of RNA interference in functional-genomics studies of a social insect. In: Sailen Barik (ed), Methods in molecular biology: siRNA, shRNA and miRNA protocols. In press.
Zhou, X. 2005. Western corn rootworm, Diabrotica virgifera virgifera, a model system for the study of insecticide resistance. pp. 250-268. In: T. X. Liu and L. Kang (eds), Latest progress in the entomological research, Science Press, Beijing, China.
Zhou, X. 2000. Cultural Entomology, the legacy of Chinese fighting crickets. pp. 646-712. In: H. S. Xie [ed]. Encyclopedia of pets. China Agricultural Press, Beijing, China.
Zhou, X., H. Cheng and P. Y. Yu. 1997. Scanning electron microscopy of sensillae on cerci and genitalia of adult Locusta migratoria manilensis (Meyen) (Orthoptera: Locustidae), pp. 118-121. In: D. R. Zhou (ed), Advanced technologies of plant protection, Science and Technology Press of China, Beijing, China.

BOOK

Wang, Y. and X. Zhou. 1996. Cultural Entomology: Insects in Art. China Agricultural Press, Beijing, China. 289 pp.

NON-REFEREED PUBLICATIONS

Scharf, M.E. and X. Zhou. 2005. Correspondence to the editor, Termite sociogenomics: a growing field. Nature Reviews Genetics. ( http://www.nature.com/nrg/journal/v6/n4/corres/nrg1575_fs.html)
Meinke, L. J., B.D. Siegfried, M.E. Scharf, S. Parimi, X. Zhou, R.J. Wright, J.A. Stebbing, and L.D. Chandler. 2003. Insecticide resistance studies target the need for alternative technologies. Report to the Areawide Corn Rootworm Project: A cooperative Approach to Management. pp. 10-11. Published by IDEA: Information Development Expanding Awareness. IDEA is affiliated with Iowa State University Extension.
Zhou, X., M.E. Scharf, L. J. Meinke, L. D. Chandler, and B. D. Siegfried. 2002. Biochemical and molecular examination of esterase-mediated insecticide in Nebraska western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae). Report to the NCR-46 Corn Rootworm Committee. pp. 39-41. Indianapolis, Indiana.
Zhou, X., M.E. Scharf, B. D. Siegfried, L. J. Meinke, R. J. Wright, and L. D. Chandler. 2000. Partial purification and characterization of the resistance-associated esterases from western corn rootworm, (Coleoptera: Chrysomelidae). Report to the NCR-46 Corn Rootworm Committee. p. 41. Madison, Wisconsin.
Scharf, M.E. Zhou, X., L. J. Meinke, B. D. Siegfried, R. J. Wright, and L. D. Chandler. 1999. Insecticide resistance monitoring in areawide and Nebraska western corn rootworm populations. Report to the NCR-46 Corn Rootworm Committee. pp. 31-36. Minneapolis, Minnesota.
Zhou, X. and X. Z. Sun. 1994. Laboratory Manual for Horticultural Entomology. China Agricultural University Press, Beijing, China. 33 pp.
Zhou, X. and X. Z. Sun. 1992. Laboratory Manual for Agricultural Entomology. 2^nd ed. China Agricultural University Press, Beijing, China. 30 pp.